Kiryl Piatkevich lab ()

Viral Vectors Packaged from Kiryl Piatkevich lab Plasmids

We are excited to present our collaboration with Kiryl Piatkevich lab. In this partnership, Kiryl Piatkevich lab provide expertly designed plasmids, and Brain Case offers high-quality virus packaging services for these plasmids.

1. FRCaMPi
FRCaMPi is a red fluorescent, genetically encoded calcium indicator optimized for enhanced in vivo applications.

Performance vs jRGECO1a:

▪Up to 2× higher dynamic range and peak ΔF/F₀ per action potential
▪40% higher signal-to-noise ratio (SNR) in wide-field cortical imaging
▪2–3× higher automated segmentation efficiency
▪50% more responsive cells detected
▪Reduced spurious neuronal correlations for cleaner data

Applications:

▪In vivo imaging of mouse somatosensory and visual cortex
▪High labeling density (80–90%) imaging
▪Single-neuron resolution analysis in densely labeled tissues
▪Accurate large-scale neuronal activity mapping

Product Number	Backbone
BC-4814	rAAV-CaMKIIα-NES-FRCaMPi
BC-4390	rAAV-hSyn-DIO-NES-FRCaMPi
BC-4389	rAAV-hSyn-DIO-NES-SomaFRCaMPi
BC-4184	rAAV-hSyn-NES-FRCaMPi
BC-4183	rAAV-CAG-DIO-NES-SomaFRCaMPi
BC-4182	rAAV-hSyn-NES-SomaFRCaMPi

WeKwikGene related plasmid: Bright and stable monomeric green fluorescent protein derived from StayGold

2. mBaoJin
mBaoJin is a highly stable monomeric green fluorescent protein for cellular and subcellular labeling

Key Performance:

▪Highly monomeric behavior
▪15-fold higher photostability than eGFP under typical imaging conditions
▪Rapid maturation (7.5 min half-time at 37°C);
▪~70-140% brighter than eGFP in mammalian cells;
▪50% more responsive cells
▪Excellent chemical stability for expansion microscopy;

Applications:

▪Long-term live-cell imaging
▪Super-resolution microscopy (SIM, SoRa, STED)
▪Expansion microscopy
▪Structural protein fusions (tubulin, actin, H2B, mitochondria)

Product Number	Backbone
BC-2966	rAAV-EF1α-DIO-mBaoJin
BC-3986	rAAV-CAG-DIO-mBaoJin

Additional Options:

Backbone	Description
pAAV-CAG-DIO-mito-mBaoJin-WPRE-polyA	For structural imaging of mitochondria
pAAV-CAG-DIO-axontag-mBaoJin-WPRE-polyA	For projection tracing in brain

3. RGEPOs
RGEPOs are red genetically encoded potassium indicators for monitoring of intracellular and extracellular K⁺ dynamics with single-cell resolution in vitro and in vivo

Key Performance:

▪RGEPO1 for extracellular potassium imaging has affinity of 2.4 mM and ΔF/F = 180% at [K⁺] = 50 mM;
▪RGEPO2 for intracellular potassium imaging has affinity of 43.3 mM ΔF/F = 334% at [K+] = 200 mM;
▪Compatible with green calcium indicators for dual K⁺/Ca²⁺ imaging under wide-field/confocal and two-photon microscopy;

Applications:

▪Real-time K⁺ monitoring in cultured neurons and astrocytes, acute brain slices, and awake mice;
▪Visualization of intracellular and extracellular K+ transients;

Product Number	Backbone
BC-5007	rAAV-CAG-Asp-RGEPO1
BC-5008	rAAV-CAG-RGEPO2

Additional Options:

Backbone	Description
pAAV-GfaABC1D-Asp-RGEPO1	For extracellular expression in astrocytes
pAAV-GfaABC1D-RGEPO2	For intracellular expression in astrocytes
pAAV-hSyn-Asp-RGEPO1	For extracellular expression in neurons
pAAV-hSyn-RGEPO2	For intracellular expression in neurons
pAAV-CAG-Asp-RGEPO1	For extracellular expression in mammalian cells
pAAV-CAG-RGEPO2	For intracellular expression in mammalian cells

4. hCRAFi-CCR2
hCRAFi-CCR2 is a genetically encoded green fluorescence biosensor for real-time monitoring of CCL2 and related chemokines

Key Performance:

▪High sensitivity: EC₅₀ = 2.5 nM for mCCL2 in astrocytes;

▪Large dynamic range: ~300% ΔF/F response;

▪17-20 hour continuous imaging capability;▪Includes null mutant controls for validation;

Applications:

▪Monitoring endogenous chemokine release during inflammation in cell culture;

▪Tracking immune responses in mouse blood and brain tissue;▪Studying spatial heterogeneity of immune signaling in vivo.

Backbone	Description
pAAV-GfaABC1D_hCRAFi-CCR2	For extracellular expression in astrocytes
pAAV-Syn-hCRAFi-CCR2_P2A_FusionRed	For extracellular expression in neurons

5. emiRFP670
emiRFP670 is a near-infrared fluorescent protein for optimized neuronal labeling and deep-tissue imaging applications.

Key Performance:

▪Superior brightness and photostability in neuronal applications;

▪Excellent tissue penetration, minimal autofluorescence;▪Compatible with multicolor imaging strategies.

Applications:

▪Deep-tissue neuronal labeling;

▪Multicolor imaging with cyan/green/red FPs;▪Zebrafish and mouse neuronal studies.

Backbone	Description
pAAV-CAG-emiRFP670-P2A-GFP	For strong expression in mammalian cells, including neurons.

Related Publication: Quantitative assessment of near-infrared fluorescent proteins | Nature Methods

To place an order or learn more about each product, please click on the product links or contact us directly. Brain Case ensures reliable virus production with rigorous quality control so that you can focus on your science.

Thank you for visiting, and we look forward to supporting your research.

Kiryl Piatkevich lab ()

Viral Vectors Packaged from Kiryl Piatkevich lab Plasmids

1. FRCaMPi
FRCaMPi is a red fluorescent, genetically encoded calcium indicator optimized for enhanced in vivo applications.

Performance vs jRGECO1a:

Applications:

2. mBaoJin
mBaoJin is a highly stable monomeric green fluorescent protein for cellular and subcellular labeling

Key Performance:

Applications:

3. RGEPOs
RGEPOs are red genetically encoded potassium indicators for monitoring of intracellular and extracellular K⁺ dynamics with single-cell resolution in vitro and in vivo

Key Performance:

Applications:

4. hCRAFi-CCR2
hCRAFi-CCR2 is a genetically encoded green fluorescence biosensor for real-time monitoring of CCL2 and related chemokines

Key Performance:

Applications:

5. emiRFP670
emiRFP670 is a near-infrared fluorescent protein for optimized neuronal labeling and deep-tissue imaging applications.

Key Performance:

Applications:

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Kiryl Piatkevich lab ()

Viral Vectors Packaged from Kiryl Piatkevich lab Plasmids

1. FRCaMPi FRCaMPi is a red fluorescent, genetically encoded calcium indicator optimized for enhanced in vivo applications.

Performance vs jRGECO1a:

Applications:

2. mBaoJin mBaoJin is a highly stable monomeric green fluorescent protein for cellular and subcellular labeling

Key Performance:

Applications:

3. RGEPOs RGEPOs are red genetically encoded potassium indicators for monitoring of intracellular and extracellular K⁺ dynamics with single-cell resolution in vitro and in vivo

Key Performance:

Applications:

4. hCRAFi-CCR2 hCRAFi-CCR2 is a genetically encoded green fluorescence biosensor for real-time monitoring of CCL2 and related chemokines

Key Performance:

Applications:

5. emiRFP670 emiRFP670 is a near-infrared fluorescent protein for optimized neuronal labeling and deep-tissue imaging applications.

Key Performance:

Applications:

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