Plasmid Library ()

CRISPR Library Solutions | Single-Vector & Two-Vector Plasmid Libraries

Product Number	Libray Name	Fuction	Price (USD)
BC-L-001	Human glucose metabolism gene knockout library	CRISPR-KO	1799
BC-L-002	Human Genome-Wide Two Plasmid Knockout Library (Brunello)	CRISPR-KO
BC-L-003	Human genome-activated two-plasmid library B sublibrary	CRISPRa
BC-L-004	Human genome-wide activation two-plasmid library A sublibrary	CRISPRa
BC-L-005	Human RNA-binding protein knockout single plasmid library	CRISPR-KO
BC-L-006	Human epigenetic knockout single plasmid library	CRISPR-KO
BC-L-007	Mouse metabolic knockout single plasmid library	CRISPR-KO
BC-L-008	Mouse genome-wide activation SAM three-plasmid library	CRISPRa
BC-L-012	Mouse genome-wide knockout two-plasmid library B sublibrary	CRISPR-KO
BC-L-013	Mouse genome-wide knockout two-plasmid library A sublibrary	CRISPR-KO
BC-L-014	Mouse genome-wide knockout single plasmid library B sublibrary	CRISPR-KO
BC-L-015	Mouse genome-wide knockout single plasmid library A sublibrary	CRISPR-KO
BC-L-016	Human Whole Genome Knockout Two-Plasmid Library B Sublibrary	CRISPR-KO
BC-L-017	Human genome-wide knockout two-plasmid library A sublibrary	CRISPR-KO
BC-L-018	Human genome-wide knockout single plasmid library B sublibrary	CRISPR-KO
BC-L-019	Human genome-wide knockout single plasmid library A sublibrary	CRISPR-KO
BC-L-009	Rat brain cDNA template hybrid plasmid library	cDNAtemplate	1599
BC-L-010	Mouse brain cDNA template hybrid plasmid library	cDNAtemplate
BC-L-011	Human Brain cDNA template Mixed Plasmid Library	cDNAtemplate
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CRISPR knockout libraries are commonly implemented in either single-vector or two-vector formats, and the choice between them largely depends on experimental design and practical considerations rather than a universally superior approach.

Single-vector systems integrate both Cas9 (or its variant) and gRNA within the same construct. This configuration simplifies experimental setup by requiring only one delivery step and eliminates the need for pre-existing Cas9-expressing cell lines. As a result, it is often preferred in scenarios where ease of use and streamlined workflows are priorities.

Two-vector systems, on the other hand, separate Cas9 and gRNA expression into different constructs or utilize cell lines that already stably express Cas9. This format can offer greater flexibility in certain experimental settings and may be advantageous when working with established Cas9 cell models or when optimizing specific components independently. In some cases, two-vector libraries incorporating paired gRNAs targeting the same gene can facilitate the generation of larger genomic deletions, which may enhance the likelihood of functional disruption.