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Plasmid ()

Plasmid Design and Construction

Plasmids are small, circular DNA molecules widely used in molecular biology for gene cloning, expression, and modification. They help introduce foreign genes into cells, making them essential tools for research and therapeutic applications.

At Braincase, we offer professional plasmid design and construction services to simplify your work. Instead of spending time and resources on in-house cloning, you can rely on our expert team to deliver high-quality, ready-to-use plasmids. Whether you need a simple expression vector or a complex gene-editing system, we ensure precision, reliability, and efficiency—so you can focus on your research while we handle the technical details.
 

Our Plasmid Construction Process

We follow a step-by-step approach to guarantee high-quality, fully functional plasmids:


 

Why Choose Braincase?

✔Custom Designs Each plasmid is tailored to your specific research needs.
✔Extensive Plasmid Library Access a diverse collection of pre-validated plasmids to accelerate your research and reduce development time.
✔Fast & Reliable We provide quick turnaround times to keep your projects on schedule.
✔Expert Support Our team is here to guide you through the entire process.
✔High-Quality Results We use the latest technology to ensure top-tier plasmid performance.

Design Your Plasmid

We work closely with you to understand your research goals. We consider key factors such as:

🔷Target gene sequence (if you want to overexpress a gene or element, or knockdown/knockout a gene)
💠
Promoter selection (if you want to enhance the expression specificity of the target gene)
🔷
Expression system (e.g., bacterial, yeast, mammalian)
💠
Selectable markers (e.g., reporter or tag genes such as GFP, mCherry, His, FLAG)
🔷
Multiple cloning sites
💠
Regulatory elements
 

Plasmid Preparation Services:

We provide high-quality plasmid DNA preparation services to support a wide range of molecular biology and gene delivery applications. Our services include both small-scale and large-scale plasmid purification, ensuring reliable DNA quality for downstream experiments such as cloning, transfection, viral packaging, and in vivo studies.

Customer-Provided Materials
If you are providing an AAV vector, please ship the materials strictly according to the instructions from our customer service team to avoid any delays or damage. Upon receipt of your plasmids and related materials, our QC system will initiate verification and/or correction of the plasmid backbone sequence to ensure compatibility with downstream AAV production.
 

Accelerate Your Research with Our Plasmid Library

Product Number Libray Name Fuction Price (USD)
BC-L-001 Human glucose metabolism gene knockout library CRISPR-KO 1799
BC-L-002 Human Genome-Wide Dual Plasmid Knockout Library (Brunello) CRISPR-KO
BC-L-003 Human genome-activated two-plasmid library B sublibrary CRISPRa
BC-L-004 Human genome-wide activation dual plasmid library A sublibrary CRISPRa
BC-L-005 Human RNA-binding protein knockout single plasmid library CRISPR-KO
BC-L-006 Human epigenetic knockout single plasmid library CRISPR-KO
BC-L-007 Mouse metabolic knockout single plasmid library CRISPR-KO
BC-L-008 Mouse genome-wide activation SAM three-plasmid library CRISPRa
BC-L-012 Mouse genome-wide knockout two-plasmid library B sublibrary  CRISPR-KO 
BC-L-013 Mouse genome-wide knockout two-plasmid library A sublibrary  CRISPR-KO 
BC-L-014 Mouse genome-wide knockout single plasmid library B sublibrary  CRISPR-KO 
BC-L-015 Mouse genome-wide knockout single plasmid library A sublibrary  CRISPR-KO 
BC-L-016 Human Whole Genome Knockout Two-Plasmid Library B Sublibrary CRISPR-KO
BC-L-017 Human genome-wide knockout two-plasmid library A sublibrary CRISPR-KO
BC-L-018 Human genome-wide knockout single plasmid library B sublibrary CRISPR-KO
BC-L-019 Human genome-wide knockout single plasmid library A sublibrary CRISPR-KO
BC-L-009 Rat brain cDNA template hybrid plasmid library  cDNAtemplate  1599
BC-L-010 Mouse brain cDNA template hybrid plasmid library  cDNAtemplate 
BC-L-011 Human Brain cDNA template Mixed Plasmid Library  cDNAtemplate 
Request a Quote/Explore the Library

 

Additional Services

🔗Gene Synthesis: Need a custom gene? We provide gene synthesis and optimization services to seamlessly integrate your gene into the plasmid of your choice.

🔗Plasmid Optimization: We offer sequencing services to verify plasmid construction and ensure accurate sequences, enhancing expression levels.

🔗Virus Packaging: If you need to package your plasmid into viral vectors, we offer reliable virus packaging services for efficient gene delivery.

🔗Stable Cell Line Generation: We help generate stable cell lines, simplifying your experimental workflow.

🔗Shuttle Vectors: Our shuttle vectors enable gene expression across multiple host systems, providing flexibility for your research.

🔗Shuttle Vectors: Our shuttle vectors enable gene expression across multiple host systems, providing flexibility for your research.

Whether you need a single plasmid for a small experiment or multiple vectors for large-scale studies, Braincase is your trusted partner in plasmid design and construction. Contact us today to learn more about how we can assist you with your project!

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