Fluorescent “superheroes”！ mBaoJin + mScarlet3-H (also known as mYongHong) Tool Viruses Empower Super-Resolution and High-Efficiency Imaging

The Dynamic Duo of Fluorescent Proteins, Performance Crushing Traditional Probes

01 mBaoJin: The Green Champion with High Brightness and Anti-Photobleaching

Figure 1: mBaoJin exhibits correct subcellular localization when fused with structural proteins (such as actin filaments, mitochondrial precursor sequences, H2B, keratin, α-tubulin, and β-actin).

 

02 mScarlet3-H (mYongHong): The All-Purpose Red Benchmark with Unmatched Durability

An upgraded masterpiece derived from mScarlet3 (Nat Methods. 2025 Jun;22(6):1288-1298, Nature Methods | mYongHong: The Next-Generation Ultra-Stable Monomer Red Fluorescent Protein), mYongHong redefines the standard for red fluorescent proteins with its extreme stability:
✅ Extreme Environment Tolerance: After being treated at 90°C for 1 hour, it retains 82.5% fluorescence, with a 500% increase in resistance to key CLEM reagent OsO₄, providing a signal-to-noise ratio 3 times higher than traditional probes.

✅ Anti-Photobleaching and Quenching: Its photostability meets the requirements for STED super-resolution imaging, with less than 5% fluorescence loss after 2 hours of continuous imaging, far surpassing traditional RFPs like mCherry and tdTomato.

✅ Rapid Clearing Adaptation: Supports 60°C accelerated tissue clearing, completing whole mouse brain clearing in 24 hours with 68% fluorescence retention, significantly shortening experimental timelines.

✅ Monomeric High Compatibility: Its pure monomeric structure prevents aggregation, making it highly compatible with viral vector long-term expression without interfering with normal cellular functions, ideal for neural circuit tracing and in vivo dynamic observations.

Figure 2: AAV virus-mediated expression of mYongHong in mouse neurons clearly marks the entire neuron morphology.
 

Brain Case Biotech Tool Viruses: Precision Delivery Upgraded

Leveraging years of experience in viral vector research and development, we have perfectly integrated two top-tier fluorescent proteins with highly efficient tool viruses, offering dual advantages:

01 Flexible Choice of Multiple Vectors

Recombinant Adeno-Associated Virus (rAAV): Strong neurotropism, supports various promoters like hSyn, CMV, and is suitable for neural circuit tracing and long-term in vivo imaging.

Lentivirus (LV): Wide infection spectrum, integrates into the host genome for stable fluorescence marking, ideal for cell line labeling and protein expression tracking.

Retrograde Virus (RV/AAV-Retro): Precisely enables retrograde neural circuit tracing, with dual fluorescent labeling for clear analysis of synaptic connections.


02  Customized Research Solutions
Dual Fluorescent Labeling Combination: mBaoJin (green) + mScarlet3-H (red), with non-overlapping spectra, supports multi-target tracking simultaneously and is compatible with various microscopes, including wide-field, confocal, and super-resolution.

Specific Targeting: Combines tissue-specific promoters with viral serotype selection to achieve precise labeling of specific cell types, such as neurons, glial cells, and cardiomyocytes.

 

Table 1: rAAV–mBaojin/mYongHong Product List

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