In vivo testing results of retrograde multi-synaptic tracing virus - Pseudorabies Virus (PRV)

Time：2026-04-02 14:53:40

Pseudorabies virus (PRV), specifically PRV-bartha, is widely used for retrograde tracing to analyze the regulatory network of the central nervous system (CNS) on peripheral organs. BrainCase has modified and screened the PRV virus to develop a fluorescence-enhanced version: PRV801 (green fluorescence) and PRV803 (red fluorescence). BrainCase conducts in vivo testing for each batch of PRV virus. The following are the results of the latest batch of tests.

PRV801 Peripheral Tracing Test Results in Mice

Test Virus Information
Product Name: PRV-CAG-3Gc
Product Number: BC-PRV-801-Plus
Batch Number: BC-PRV260106
Product Titer: 9.50E+09 PFU/mL

Testing Protocol
Test Site: Upper limb muscle
Test Animal: C57 Mouse

Day 0: Injection of BC-PRV260106 into the right upper limb muscle (2.5μL)
Day 4: Sampling

Injection Date: January 16, 2026
Perfusion Date: January 21, 2026

Figure 1: Surgical schematic diagram showing PRV injection into the mouse upper limb muscle

Figure 2: Representative brain regions labeled with PRV801

After injecting 2.5μL of BC-PRV260106 into the upper limb muscle, perfusion sampling was performed 5 days later. The results, shown in Figure 2, reveal that neurons in multiple brain regions, including M1 and PVN, were labeled with PRV, with strong fluorescence signals. This indicates that the virus is suitable for retrograde multi-level synaptic tracing studies.

👇Click to view the latest batch Test ReportBC-PRV-801-Plus TestReport
.pdf

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In vivo testing results of retrograde multi-synaptic tracing virus - Pseudorabies Virus (PRV)

PRV801 Peripheral Tracing Test Results in Mice

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In vivo testing results of retrograde multi-synaptic tracing virus - Pseudorabies Virus (PRV)

PRV801 Peripheral Tracing Test Results in Mice

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