PRV Neural Circuit Tracing Services, Supporting Peripheral-Central Research

1. What is Pseudorabies Virus (PRV)?

Pseudorabies virus (PRV), also known as porcine herpesvirus type 1, has a genome of approximately 150 kb linear double-stranded DNA. The Bartha strain of PRV exhibits retrogradetrans-synaptic properties. Recombinant tracing tool viruses based on the Bartha strain can strictly label neural networks through multi-level retrograde trans-synaptic pathways. After infecting neural cells, the virus replicates intracellularly and expresses target genes, with progeny viruses transporting across synapses to enter upstream neurons, initiating a new round of replication and retrograde trans-synaptic spread.

Case 1:

Figure 1: Schematic Diagram of Virus Injection
 

Figure 2: Retrograde Multi-Level Virus Tracing of Muscles (Brain Case Test Data)
 

Case 2:

·Experimental Objective: To investigate the neural circuits associated with and controlling urination, specifically which key brain regions are involved in the body's autonomous urination.

·Experimental Plan: The PRV (pseudorabies virus) tracer virus was injected in small amounts into the left and right sides of the bladder wall using PRV-531 (green) and PRV-724 (red); due to significant neurological symptoms and eventual death in animals infected with PRV, the animals were closely monitored daily to ensure recovery to a normal state post-surgery. When in a near-death condition, the animals were subjected to cardiac perfusion to extract the brain.

·Experimental Results: About five days after the virus injection, the animals exhibited notable neurological symptoms. Researchers gradually performed cardiac perfusion to extract the brain based on the observed conditions. Following the preparation of frozen sections of brain tissue, the following data was obtained. Based on the experimental results, researchers found that the primary motor cortex (M1), primary somatosensory cortex (S1), and the micturition center (PMC) have direct or indirect connections.

Figure 3: Retrograde multilevel viral tracing of bladder wall nerves (PMID: 30361547)

3. PRV Neural Circuit Tracing Services Offered by Brain Case

In recent years, the research focus on peripheral-central connections has surged, with more peripheral organs becoming the subject of investigation. Brain Case is committed to customer-oriented services, supporting research on peripheral-central neural circuits. We provide a comprehensive PRV neural circuit tracing service, including the stable older versions PRV531 (green) and PRV724 (red), as well as the efficient and bright new products PRV801 (green) and PRV803 (red), which have been successfully applied in multiple high-interest peripheral tissue studies. Below are examples of research sites we have operated on:
◆ Digestive System: Duodenum, cecum, colon, rectum, pancreas
◆ Endocrine System: Thyroid
◆ Reproductive System: Testes, ovaries, epididymis, prostate
◆ Urinary System: Bladder wall
◆ Musculoskeletal System: Muscle, bone marrow cavity
◆ Cardiovascular System: Heart
◆ Immune System: Spleen
◆ Circulatory System: Femoral cavity
◆ Nervous System: Brain + spinal cord

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For more information on related products and services, please contact: BD@ebraincase.com

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