Scientific Research Services | Brain Case Neural Circuit Tracing Service

Ⅰ. Why Conduct Neural Circuit Research?

1. In recent years, neurodegenerative diseases related to the nervous system have become increasingly common.
2. The incidence of neurological disorders is growing year by year, leading to a steadily increasing economic burden on society.
3. Neurological and psychiatric disorders are closely related to abnormalities in neural circuits: conducting neural circuit research helps to reveal the mechanisms of brain function; it aids in identifying potential biomarkers for diseases such as depression, schizophrenia, and autism, which can be used for early diagnosis and monitoring of these conditions; by understanding the signal transmission and coding mechanisms of neural circuits in the brain, the performance and reliability of brain-computer interfaces can be improved, enabling more natural and efficient   human-computer interaction.

Ⅱ. How Can Brain Case Biotech Support Neural Circuit Research?

Animal Experiment Services


Animal Platform — High-Priority Research Topics on Circuits

 

Ⅲ. Why choose Brain Case?
We are a research team from the Chinese Academy of Sciences with extensive experience in viral development. Every critical step is strictly overseen by professional teams, including BD, SD, PM, and QA experts. We have SPF-grade animal facilities and P2 laboratories, equipped with state-of-the-art research equipment, including high-resolution microscopes, cell culture facilities, and molecular biology laboratories.
We offer highly customized services to meet the specific needs of different clients, emphasizing communication and feedback to ensure service transparency and customer satisfaction. We adhere strictly to ethical and scientific standards, ensuring the quality and compliance of our research.

Experimental Project Service Process

 

Ⅳ.  Case Study

1. Sparse Labeling (CNS)

• Virus Information: CSSP-YFP-8E3
• Injection Dose: 100 nl
• Animal Information: Thy1-Cre Mice
• Injection Site: Primary Motor Cortex M1
• Expression Period: 3 weeks
• Sample Information: Frozen Sections, 50 µm Thickness

2. Retrograde Labeling (CNS)

• Virus Information: rAAV2/Re-CaMKIIα-EGFP
• Injection Dose: 200 nl
• Animal Information: C57 Mice
• Injection Site: BPn
• Expression Period: 3 weeks
• Sample Information: Frozen Sections, 50 µm Thickness

3. Retrograde Mono-synaptic Tracing (CNS)

• Virus Information: rAAV-EF1α-DIO-EGFP-T2A-TVA, rAAV-EF1α-DIO-N2cG, rAAV-hSyn-SV40 NLS-Cre, CVS-EnvA-G-△tdTomato
• Animal Information: C57 Mice
• Injection Site: Primary Motor Cortex M1
• Injection Dose: Helper Virus TVA: RVG: Cre = 1:2:1 (Mixed Injection 100 nl), RV injected after 14 days of expression
• RV Infection Period: 7 days
• Sample Information: Frozen Sections, 50 µm Thickness

4. Retrograde Muti-synaptic Tracing (Peripheral- Central nervous system)

• Virus Information: PRV-CAG-EGFP, PRV-CAG-3MS
• Injection Dose: 3 µl (each)
• Animal Information: C57 Mice
• Injection Site: Bilateral Leg Muscles
• Expression Period: Approximately 6 days
• Sample Information: Frozen Sections, 40 µm Thickness

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