This AAV expresses hChR2(H134R)-EGFP driven by an astrocyte GfaABC1D promoterThe GfaABC1D promoter is a truncated GFAP promoter.
Channelrhodopsins are light-gated ion channels that exist naturally in microalgae. hChR2 is a humanized version of ChR2 formammalian expression.ltis maximally excited by 470 nm light. The wild-type, as well as a few mutations, provide the fastest excitalionof the channelrhodopsins offered, and are widely used in optogenetics techniques in neuroscience. Due to delayed channel closure,hChR2(H134R) is a gain-of-function mutation that produces larger photocurrents than wild-type hChR2 but slows down channelkinetics.
EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria, with aExcitation/Emission speclrum around 488nm/507nm, ltis reported to be a rapidly maturing weak dimer with moderate acid sensitivily.
Forresearch use only.
| Overview |
| Functional Gene |
hChR2(H134R) |
| Promoter |
GfaABCID |
| Tag |
EGFP |
| Serotype |
AAV1; AAV5; AAV8; AAV9; AAV.PHP.eB; AAV2;
AAV6; AAV.rhW; AAV.DJ; AAV.DJ8; AAV.PHP.B;
AAV.PHP.S; AAV2/RETRO; AAV.anc80 |
| Virus Type |
Adeno-associated Virus (AAV) |
| Applications |
Activating optogenetic |
| Storage |
Should be stored at -80° C |
