Brain Case Research Services | PRV Neural Circuit Tracing Service Empowering Peripheral–Central Research

1.What is Pseudorabies Virus (PRV)?

After infecting neurons, PRV replicates intracellularly and expresses the target gene. The progeny virus is transported to the postsynaptic terminal and then crosses the synapse retrogradely to infect upstream neurons, initiating a new round of replication and retrograde trans-synaptic transmission. Pseudorabies virus (PRV), also known as Suid herpesvirus type I, is a linear double-stranded DNA virus with a genome of approximately 150 kb. The Bartha strain of PRV possesses only retrograde, trans-synaptic properties. Recombinant tracing viral tools derived from the Bartha strain can label neural networks strictly in a retrograde, multi-synaptic manner.

2. What Can PRV Do in Neural Circuit Research?

Case 1

Experimental Objective:

Experimental Design:

Figure 1: Schematic diagram of viral injection
 

Experimental Results:

Figure 2: Multisynaptic retrograde viral tracing of muscle (Brain Case Test Data)
 

Case 2

Experimental Objective:

To investigate the neural circuits involved in the control of urination and identify the key brain regions that regulate voluntary micturition.

Experimental Design:

PRV viral tracers were used. Microinjections of PRV-531 (green) and PRV-724 (red, expressing mRuby3) were administered into the left and right sides of the bladder wall, respectively. Because PRV infection causes severe neurological symptoms leading to death, animals were closely monitored at high frequency to ensure normal recovery after surgery. Cardiac perfusion and brain collection were performed when animals reached a moribund state.

Experimental Results:

Approximately 5 days post-injection, animals developed distinct neurological symptoms. Researchers then carried out cardiac perfusion and brain collection as appropriate. Frozen brain sections provided the following data: results revealed that the primary motor cortex (M1), primary somatosensory cortex (S1), and the pontine micturition center (PMC) are directly or indirectly connected to bladder control.

Figure 3. Multisynaptic retrograde viral tracing of bladder wall innervation (PMID: 30361547)
 

3. PRV Neural Circuit Tracing Services Offered by Brain Case

In recent years, research on peripheral–central neural circuits has gained increasing attention, with more peripheral organs becoming key study targets. Brain Case is committed to providing customer-oriented solutions to support peripheral–central neural circuit research. We offer a comprehensive one-stop PRV neural circuit tracing service, including stable legacy tracers PRV-531 (green) and PRV-724 (red), as well as highly efficient and bright new tracers PRV-801 (green, expressing mClover) and PRV-803 (red). These products have been successfully applied to studies of various peripheral tissues.

Examples of research sites we have worked on include:
Digestive system: duodenum, cecum, colon, rectum, pancreas
Endocrine system: thyroid
Reproductive system: testes, ovaries, epididymis, prostate
Urinary system: bladder wall
Musculoskeletal system: muscles, bone marrow cavity
Cardiovascular system: heart
Immune system: spleen
Circulatory system: femoral cavity
Nervous system: brain and spinal cord
 

Product List:

✅ Click to View Product Details:  
BC-PRV-531,   BC-PRV-803,   BC-PRV-531-Plus,   BC-PRV-801-Plus,
BC-PRV-803-Plus,   BC-PRV-531-Pro,   BC-PRV-803-Pro

Contact US

If you are interested in neural circuit tracing research and would like to learn more about practical applications, specific case studies, or the principles of viral tools and their use in central–peripheral interaction studies, please contact BD@ebraincase.com

 

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