The neurotransmitter sensor constructed based on the GPCR activation principle is named GRAB sensor, a type of genetically encoded high spatiotemporal resolution neurotransmitter indicator
Calcium signal recording technology detects the Ca2+ concentration in cells and converts it into fluorescence signals, transfers cell electrical activity into light signals. It uses genetically encoded calcium ion indicators GECIs, such as GCaMP.
Chemical genetics, or pharmacogenetics, are non-invasive method of neural function manipulation. DREADDs technology refers to the activation or inhibition of DREADDs receptors by only the specific drug Clozapine-N-oxide (CNO).
optogenetics, optogenetic tools, channelrhodopsin, light-gated neural control, optogenetic AAV vectors
CRISPR system is for gene editing. The sgRNA (small guide RNA) recognizes and binds to the target sequence guiding the Cas protein to cut at the binding site, resulting in a double-strand break (DSB) in the DNA, mediating CRISPR-Cas9 gene editing.
RNAi is highly efficient and specific inhibitor of gene expression, using viral vectors to mediate shRNA(short hairpin RNA) interference can regulate gene expression, and then study gene function
