Neural circuit studies ()

Introduction

The brain is the most complex system in the body, determining physiological activities such as emotion, memory, and learning. Neural circuits are the basis for brain functions. Clarifying the composition and working principles of neural circuits is of great significance for understanding, utilizing and protecting the brain. At present, tool viruses are widely used in anatomical research on central and peripheral nerves and play an important role in the analysis of neural circuit structure and function. After years of hard work, the Brinkes team has completed about 100 animal experimental services for nearly 80 scientific research units, built a rat and mouse neural circuit tracing and neural function research platform, and has professional behavioral experimental equipment and recording analysis systems. , can provide various types of experimental services in rats and mice, such as central nervous circuit labeling and functional research, peripheral-central circuit labeling experiments, etc.

1. Application of peripheral-central nervous circuit labeling

1. Muscle-center (nervous system-related diseases)

(Yujie Guo, J Neurosci, 2018)

2. Heart-center (cardiovascular disease)

(Yuhong Wang, Front Cardiovasc Med, 2021 )

3. Liver-center (metabolism-related research)

(Sarah Stanley, Proc Natl Acad Sci USA, 2010)

4. Pancreas-center (diabetes)

(Wilfredo Rosario, Diabetes, 2016)

5. Bladder-center (overactive bladder syndrome)

(Jiwei Yao, Nat Neurosci, 2018)

6. Gonads-center (sexually related diseases)

(P James, J Neuroendocrinol, 2008)

7. Spleen-center (research on immunity)

(Xu Zhang, Nature, 2020)

2. Applications of central nervous system neural circuit tracing and labeling

1. Label GABAergic neurons in hippocampal pyramidal neurons that act on both mPFC and ACB

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2. Retrograde tracing of RV in OSNs

(Niccolò Zampieri, Neuron, 2014)

3. Tracing of third-level projection circuits of amygdala, parafascicular nucleus and second somatosensory cortex

(Zhu et al., Cell Reports, 2019)

3. Research on neural circuit functions

1. Optogenetics

(Zhang et al., Neuron, 2023)

2. Chemical geneticsxxx

(Xi et al., Sci. Adv. 2023)

3. Calcium imaging

(Liu et al., Neuron, 2023)

One-stop-outsourcing service process:

• ①Project research, background research
• ②Sign the contract and confidentiality agreement
• ③Experimental plan design, evaluation and determination
• ④Carry out experiments and report regularly
• ⑤ Submit original information and data

(Advantage)

professional team

The core research team of brain science of the Chinese Academy of Sciences
has rich experience in the development and application of virus tools.

experiment platform

The two major experimental bases in Wuhan and Shenzhen
have SPF-level animal rooms, P2 laboratories, and complete three-dimensional imaging systems.

disease animal models

Tumor animal models
Nervous system disease models
Metabolic disease models
Other common animal disease models

Virus category

More than 20 neurotropic viruses